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Image Search Results
Journal: Current protocols in stem cell biology
Article Title: Spatiotemporal control of morphogen delivery to pattern stem cell differentiation in three-dimensional hydrogels
doi: 10.1002/cpsc.97
Figure Lengend Snippet: Setup for dual delivery of morphogen-containing media. (A) A large polystyrene box is used to hold two 50mL conicals. The figure shows example drill locations for connecting tubing to external peristaltic pumps. (B) An assembled perfusion device with two 50mL conical tubes and bioreactor polystyrene box. (C) An illustration of the perfusion setup shows how the media is drawn from a conical tube reservoir and pushed around a pumphead, through the gel, and back into the reservoir. Figure 3C is modified from O’Grady et al. (2019) and is used under the terms of the Creative Commons Attribution License (CC-BY 4.0).
Article Snippet: Creo Parametric (Parametric Technology Corporation, Boston, MA, USA) 3D printer Acrylonitrile butadiene styrene (ABS—ESD7, Stratasys, Rehovot, Israel) Drill (DR260, Black and Decker, New Britain, CT, USA) Plastic cutting drill bit (27465A84, McMaster-Carr, Elmhurst, IL, USA) Female Luer bulkheads (EW-45508–30, Cole-Parmer, Vernon Hills, IL, USA) Locking nuts (SI-45509–04, Cole-Parmer, Vernon Hills, IL, USA) Square stock (6527K174, McMaster-Carr, Atlanta, GA, USA) Polydimethylsiloxane kit (Sylgard 184, Dow Corning, Midland, MI) Oven Bleach Ethanol Deionized water Autoclave 1/16” Silicon tubing (EW-95802–02, Cole Parmer, Vernon Hills, IL, USA)
Techniques: Modification
Journal: Current protocols in stem cell biology
Article Title: Spatiotemporal control of morphogen delivery to pattern stem cell differentiation in three-dimensional hydrogels
doi: 10.1002/cpsc.97
Figure Lengend Snippet: Troubleshooting
Article Snippet: Creo Parametric (Parametric Technology Corporation, Boston, MA, USA) 3D printer Acrylonitrile butadiene styrene (ABS—ESD7, Stratasys, Rehovot, Israel) Drill (DR260, Black and Decker, New Britain, CT, USA) Plastic cutting drill bit (27465A84, McMaster-Carr, Elmhurst, IL, USA) Female Luer bulkheads (EW-45508–30, Cole-Parmer, Vernon Hills, IL, USA) Locking nuts (SI-45509–04, Cole-Parmer, Vernon Hills, IL, USA) Square stock (6527K174, McMaster-Carr, Atlanta, GA, USA) Polydimethylsiloxane kit (Sylgard 184, Dow Corning, Midland, MI) Oven Bleach Ethanol Deionized water Autoclave 1/16” Silicon tubing (EW-95802–02, Cole Parmer, Vernon Hills, IL, USA)
Techniques: